Scientist I / II, Functional Genomics, Research
We are seeking a highly motivated, innovative and team-oriented individual to join the Human Genetics research group. Within the Human Genetics group, the Functional Genomics group is expanding in order to carry out its mission of applying genetic screens and other functional genomic technologies to discover and validate novel therapeutic targets. The successful Functional Genomics candidate will utilize the latest technologies to advance our therapeutic research and discovery efforts. Expertise should include the use of high throughput gene editing technologies and next generation sequencing to assess genomic functions. The position requires adaptability, creativity, and a capacity for constant innovation in pursuit of company goals.
Responsibilities will depend on the applicant’s skills but may include:
- Design and execute CRISPR and/or RNAi screens to for target identification, MOA characterization and other functional characterizations.
- Develop phenotypic and functional screening assays in collaboration with cross-functional teams by generating cell lines, developing lentiviral libraries, and utilizing RNP based transfections to study genetic variants in the context of disease biology.
- Perform pooled and arrayed based gene editing screens with genomics-based assays (RNAseq, scRNA-seq) and data analysis (R or Python).
- Work with NGS and bioinformatics scientists to process genomic samples, analyze screening results, and generate screening hits for follow-up studies.
- Design and perform tiling gene editing screens to identify causal SNPs and genes from GWAS human genetic studies.
- Design, develop, execute, and analyze experimental research projects in collaboration with cross-functional teams and contract research organizations.
- Design and execution of cellular studies and screens using CRISPR/Cas9, RNAi, or other genomic perturbation techniques.
- Experience with cellular models of disease utilizing techniques such as hESC/hiPSC differentiation and organoid cultures.
- Generation and interpretation of epigenetic datasets (ATAC-seq, ChIP-seq, SELEX-seq) and massively parallel reporter assays for mapping regulatory elements.
- Working knowledge in genomics technology (NGS, RNA-seq, single cell seq) and with the use of liquid handling robotics for cell culture or NGS library preparation is preferred.
- Prior laboratory experience with mammalian cell culture, cell and molecular biology and biochemical techniques.
- Ph.D. in Molecular/Cell Biology, Genomics, Bioengineering, or related discipline with post-doctoral research experience in either an academic or industry lab.
- Strong analytical, written, and verbal communication skills.
- Track record of productivity and effective projects.
- Strong interest implementing new laboratory techniques and protocols. Attention to detail, time management and organization skills are critical.