Scientist/Senior Scientist, Protein Engineering and Molecular Sciences

Research & Development Cambridge, Massachusetts


Description

Company Overview

Repertoire Immune Medicines is a clinical-stage biotechnology company working to unlock and direct the remarkable power of the human immune system to prevent or treat cancer, as well as autoimmune and infectious disease.  The company is founded on the belief that the repertoire of T cell receptor (TCR)-antigen codes that drive health and disease represents one of the greatest opportunities for innovation in medical science.  Repertoire scientists created and developed the DECODE and DEPLOY suite of technologies, which allow in-depth characterization of TCR-antigen pairs, and the ability to rationally design and develop novel targeted immune medicines.

From its sites in Cambridge, Mass., and Zurich, Switzerland, Repertoire’s team of more than 120 is creating a new category of immune medicines enabled from its DECODE discovery platform.  The company’s first product in the clinic is a novel multiclonal T cell therapy consisting of T cells derived from the peripheral blood, activated against a curated set of antigens, and armed with a proprietary cytokine.  Repertoire is in clinical-stage immuno-oncology development today, targeting metastatic solid tumors.  In the field of autoimmunity, Repertoire is currently using its proprietary platform to discover novel epitopes and TCRs from patients with type 1 diabetes.  The company is also using its DECODE platform to discover infectious disease antigens and responding T cell clones.

Role Overview

We are seeking a Scientist/Senior Scientist to join the protein engineering and molecular sciences team to support the discovery and development of novel protein therapeutics cross-functionally with the pharmacology and preclinical development team.  The position will focus on the design, generation, optimization, and characterization of protein-based modalities to enable the development of effective therapies for immune-related disorders including autoimmune diseases and cancer.  The Scientist/Senior Scientist will make significant impact on projects and the achievement of group goals predominantly through hands-on experimentation and data analysis.  The candidate will collaborate with multi-disciplinary teams to prioritize hits for protein generation and coordinate biological characterization of molecules.  The ideal candidate will be a motivated, curious, and innovative scientist with a proven ability to solve complex problems.

Responsibilities

  • Lead protein engineering efforts in the design of next-generation biologics including management of protein production CRO’s.
  • Characterize target specificity and activity of lead molecules with cell binding and cell signaling assays using flow cytometry.
  • Evaluate affinity and kinetic characterization of lead molecules via immobilized protein assays (e.g. BLI, SPR).
  • Custom ELISA design and development for quantification of lead molecules from serum, plasma, and tissue culture samples.
  • Assess protein quality attributes, such as purity and homogeneity, through assays including SDS-PAGE and SE-HPLC.
  • Development of functional assays to evaluate biological effectiveness of engineered molecules.
  • Independently design and execute experiments, analyze data, and troubleshoot methods.
  • Maintain up-to-date electronic lab notebook.
  • Coordinate with cross-functional teams on the biological characterization of designed molecules; build consensus around candidates and influence decision making through the assessment of data and presentation of results.
  • Communicate experimental design and results to team members and present updates to department.

Qualifications

  • Ph.D. in Biochemistry, Chemistry, Molecular Biology, Structural Biology, or related discipline with 2-8 years post-graduate experience in industry or academia.
  • Detailed knowledge of and experience in protein design and engineering.
  • Must have experience with analysis of protein-protein interactions using BLI or SPR.
  • Well-versed in molecular biology, cloning, subcloning, and construct design with extensive hands-on experience.
  • Experience with custom ELISA generation.
  • Skilled in multi-color flow cytometry.
  • Experience with antibody engineering and/or biophysical characterization.
  • Experience with mammalian protein expression.
  • Extensive experience and track record in protein purification (affinity, IEX, SEC) with demonstrated ability to deliver high-quality, purified protein.
  • Familiarity with or experience in any of the following is a plus:
    • Protein analytical and biophysical techniques including HPLC, DLS, SEC-MALS.
    • Antibody characterization including binding affinity, stability, epitope binning.
    • Multi-specific antibody or bioconjugate generation and characterization.
  • Ability to work in a fast-paced and collaborative environment.
  • Results-oriented, team player.
  • Strong written and oral communication skills.

Repertoire is committed towards social responsibility and developing an inclusive culture.  Much as the power of the immune system lies in the diversity of T and B cells, we believe that our work requires the creativity and ingenuity of a diverse workforce, and we are committed to pursuing that in all facets of the work experience at Repertoire.  We will continue to educate ourselves about the inequities and barriers present in our society and act as a company where we can make a difference.

Repertoire is proud to be an Equal Opportunity Employer.