Scientist/Sr. Scientist: Antigen Delivery

Research & Development Cambridge, Massachusetts


Description

Repertoire is seeking a highly motivated Scientist or Senior Scientist to join the Cell and Protein
Development team. This team is responsible for innovation and early development of RIM’s
best-in-class natural T-cell therapy. The hire will have the opportunity to substantially
contribute to the potency of the Repertoire clinical product by applying state-of-the-art antigen
delivery methods within our T-cell manufacturing process . Specifically, the successful hire will
develop technologies for optimizing expression of tumor antigens in dendritic cells to maximize
T-cell quality and quantity. These technologies include methods of antigen delivery, DC/T-cell
co-culture, antigen-specific T-cell enrichment, and analytical assessment of DC and T-cell
quality. The hire will work within a team of cell-process engineers, cell therapy experts, and
analytical scientists to assess, understand, and improve the function of RIM’s unique class of Tcell
therapy in collaboration with Translational, Process Development, and Analytic teams at
Repertoire.

The new hire will:
• Contribute to the creation of best-in-class processes for making tumor-reactive T-cells
• Apply analytical methods to characterize peptide repertoires on immune cells
• Help design mRNA constructs for tumor-antigen expression in immune cells
• Optimize and scale mRNA transfection methods for GMP manufacturing
• Assess different methods of antigen-delivery and presentation on T-cell function
• Contribute to new T-cell/DC co-culture technologies to improve reactivity, phenotype,
and yield of the RIM T-cell product
• Use strong verbal and written communication skills to communicate scientific and
project updates to leadership
• Successfully collaborate with CMC and Analytic Development teams to innovate and
implement new T-cell and DC processes

The ideal candidate will have a strong background in a few of the following:
• Dendritic cell biology and characterization including methods of antigen uptake and
peptide display
• Nucleic acid (particularly mRNA) transfection techniques
• Dendritic cell and T-cell co-culture for the expansion of antigen-reactive sub-populations
• Appropriate analytics to assess DC and T-cell phenotype and function
• Collaborative work within and across teams