Research Associate, Protein Purification

Research & Development Cambridge, Massachusetts


Company Overview

Repertoire Immune Medicines is a clinical-stage biotechnology company working to unlock and direct the remarkable power of the human immune system to prevent or treat cancer, as well as autoimmune and infectious disease. The company is founded on the belief that the repertoire of T cell receptor (TCR)-antigen codes that drive health and disease represents one of the greatest opportunities for innovation in medical science. Repertoire scientists created and developed the DECODE and DEPLOY suite of technologies, which allow in-depth characterization of TCR-antigen pairs, and the ability to rationally design and develop novel targeted immune medicines.

From its sites in Cambridge, Mass., and Zurich, Switzerland, Repertoire’s team of more than 120 is creating a new category of immune medicines enabled from its DECODE discovery platform. The company’s first product in the clinic is a novel multiclonal T cell therapy consisting of T cells derived from the peripheral blood, activated against a curated set of antigens, and armed with a proprietary cytokine. Repertoire is in clinical-stage immuno-oncology development today, targeting metastatic solid tumors. In the field of autoimmunity, Repertoire is using its proprietary platform to discover novel epitopes and TCRs from patients with type 1 diabetes. The company is also using its DECODE platform to discover infectious disease antigens and responding T cell clones.

Role Overview

We are seeking a passionate scientist who loves working with proteins to uncover their mysteries.  The ideal candidate will have hands-on experience with cell culture and protein production, and the capability and desire to learn and apply new experimental methodologies.  The incumbent will work at the bench to express, purify, and QC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, and analytical methods such as FPLC and HPLC.

Key Responsibilities

  • Express recombinant proteins from mammalian and bacterial cultures.
  • Execute large-scale protein purifications using immobilized metal affinity, ion-exchange, and size exclusion chromatography.
  • Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, ELISA, and other biochemical/biophysical assays to determine protein purity and function.
  • Capture primary data and document work as required in Electronic Laboratory Notebook (ELN) maintaining up to date records.
  • Present data routinely to the Protein Sciences group and other multidisciplinary groups.


  • B.S./M.S. in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
  • 0-2 years of academic or biotech/pharma laboratory experience.
  • Experience with recombinant protein expression and purification using mammalian cell lines and/or E. coli.
  • Experience with FPLC systems and with affinity, IEX, and SEC protein purification methods is desirable.
  • Experience with general in vitro cell culture/immunology techniques and culture of primary cell lines a plus.
  • Strong organizational and communication skills along with excellent attention to detail.
  • Ability to work in a fast-paced and quickly changing environment.

Repertoire is committed towards social responsibility and developing an inclusive culture. Much as the power of the immune system lies in the diversity of T and B cells, our work requires the creativity and ingenuity of a diverse workforce.  We believe in actively pursuing equity in all facets of the work experience at Repertoire.  We will continue to educate ourselves about the inequities and barriers present in our society and take action as a company where we can make a difference.

Repertoire is proud to be an Equal Opportunity Employer.