Research Associate I or II – Protein Engineering & Molecular Sciences

Research & Development Cambridge, Massachusetts


Description

Company Overview

Repertoire Immune Medicines is a preclinical-stage biotechnology company working to unlock and direct the remarkable power of the human immune system to prevent or treat cancer, as well as autoimmune and infectious disease.  The company is founded on the belief that the repertoire of T cell receptor (TCR)-antigen codes that drive health and disease represents one of the greatest opportunities for innovation in medical science.  Repertoire scientists created and developed the DECODE suite of technologies, which allow in-depth characterization of TCR-antigen pairs, and the ability to rationally design and develop novel targeted immune medicines.

From its sites in Cambridge, Mass., and Zurich, Switzerland, Repertoire’s team of more than 80 is creating a new category of immune medicines enabled from its DECODE discovery platform.  Repertoire is currently using its proprietary platform to discover novel epitopes and TCRs from patients with autoimmune diseases such as type 1 diabetes and multiple sclerosis as well as to enable development of immune oncology based therapeutics to treat solid tumors. 

Role Overview

We are seeking a motivated Research Associate I or Research Associate II to work in the Protein Engineering and Molecular Sciences (PE&MS) team to support the discovery and development of protein therapeutics.  The candidate will play an integral role in discovering novel therapeutics through yeast display-based protein engineering.  The candidate will perform all techniques associated with yeast display, including culture and induction of yeast, transformation of yeast with DNA-based libraries, and phenotypic selection using fluorescence activated cell sorting (FACS) or magnetic activated cell sorting (MACS).  The ideal candidate will be proficient with standard molecular biology techniques, such as PCR, primer design, and DNA preparation for sequencing, as well as have conceptual knowledge of protein display technologies.  The candidate will be responsible for experimental design, implementation, data analysis, and presenting data to their supervisor and to the team in an effective and timely manner.  Drive to execute, experimental rigor, and scientific curiosity are essential for this role.  The candidate will have strong interpersonal, verbal, and written communication skills that will enable them to integrate with our highly cross-functional teams. 

Key Responsibilities

  • Work with the PE&MS team to design and execute experimental projects under the supervision of direct manager and scientific project leads.
  • Use recombinant protein display systems (e.g. yeast, mammalian, or phage display) for various protein engineering applications.
  • Use molecular biology techniques (e.g. DNA isolation and purification from yeast and/or E. coli, PCR, DNA transformation of yeast and/or E. coli) to design and construct DNA libraries.
  • Use FACS and MACS to enrich libraries of displayed molecules for phenotypes of interest.
  • Maintain and implement good laboratory practices including accurate laboratory notebook (ELN) documentation, instrument and reagent validation, and adherence to lab safety policies.
  • Maintain archive of cell stocks and plasmids.
  • Analyze and interpret experimental data both upstream and downstream of protein engineering efforts.

Qualifications/Experience

  • B.S. or M.S. in Biochemistry, Biology, Bioengineering, Immunology, or a similar field.
  • 0-2+ years of academic or biotech/pharma laboratory experience.
  • Experience with protein display technologies preferred, but not required (e.g., yeast, phage).
  • Demonstrated understanding of common molecular biology techniques is highly preferred (e.g., PCR, primer design, DNA purification, DNA library construction, maintenance and culture of yeast and E. Coli).
  • Ability to identify, troubleshoot, and solve critical issues related to experimental protocols.
  • Strong organizational skills, excellent attention to detail, and data analysis skills are required.
  • Must work well independently as well as an integral part of the team.
  • Ability to work in a fast-paced and quickly changing environment.
  • Motivated by driving the generation of experimental data at the bench.
  • Knowledge of oncology or immunology is beneficial.
  • Proficiency with Microsoft Excel, PowerPoint, Word required and experience with FlowJo and SnapGene preferred.
  • Desire and ability to quickly learn new lab techniques and understand the significance to program goals.
  • Strong written and oral communication skills are required.

 

Repertoire is committed towards social responsibility and developing an inclusive culture.  Much as the power of the immune system lies in the diversity of T and B cells, we believe that our work requires the creativity and ingenuity of a diverse workforce, and we are committed to pursuing that in all facets of the work experience at Repertoire.  We will continue to educate ourselves about the inequities and barriers present in our society and act as a company where we can make a difference.

Repertoire is proud to be an Equal Opportunity Employer.