Research Associate I/II, Protein Engineering And Molecular Sciences

Research & Development Cambridge, Massachusetts


Company Overview

Repertoire Immune Medicines is a clinical-stage biotechnology company working to unlock and direct the remarkable power of the human immune system to prevent or treat cancer, as well as autoimmune and infectious disease.  The company is founded on the belief that the repertoire of T cell receptor (TCR)-antigen codes that drive health and disease represents one of the greatest opportunities for innovation in medical science.  Repertoire scientists created and developed the DECODE and DEPLOY suite of technologies, which allow in-depth characterization of TCR-antigen pairs, and the ability to rationally design and develop novel targeted immune medicines.

From its sites in Cambridge, Mass., and Zurich, Switzerland, Repertoire’s team of more than 120 is creating a new category of immune medicines enabled from its DECODE discovery platform.  The company’s first product in the clinic is a novel multiclonal T cell therapy consisting of T cells derived from the peripheral blood, activated against a curated set of antigens, and armed with a proprietary cytokine.  Repertoire is in clinical-stage immuno-oncology development today, targeting metastatic solid tumors.  In the field of autoimmunity, Repertoire is using its proprietary platform to discover novel epitopes and TCRs from patients with type 1 diabetes.  The company is also using its DECODE platform to discover infectious disease antigens and responding T cell clones.

Role Overview

We are seeking a Research Associate I/II to join the Protein Engineering and Molecular Sciences team to support the discovery and development of novel protein therapeutics.  The position will focus on supporting the generation, optimization, and characterization of protein-based modalities to enable the development of effective therapies for immune-related disorders, including autoimmune diseases and cancer.  The Research Associate I/II will make significant impact on projects and the achievement of group goals through hands-on experimentation and data analysis.  The candidate will work closely with team members and collaboratively with multi-disciplinary teams.  The ideal candidate will be results-oriented and motivated by driving the generation of experimental data at the bench.

Key Responsibilities

  • Work with the team to design and execute experimental projects under the supervision of the direct manager and scientific project leads
  • Maintain mammalian cells for transient transfection
  • Perform molecular biology/cloning and plasmid preparation. Maintain plasmid and protein inventory.
  • Perform mammalian and bacterial protein expression
  • Support and perform protein purification to deliver high-quality proteins to teams
  • Execute protein characterization including: SDS-PAGE, Western blotting, spectrophotometry, endotoxin analysis and SE-HPLC
  • Contribute to protein production method development and optimization
  • Perform biophysical characterization, including thermostability analysis and target binding assessment by biolayer interferometry and flow cytometry
  • Record results using best practices in an electronic lab notebook
  • Communicate experimental design and results to team members and present updates to department
  • Support general lab operations with reagent preparation, instrument maintenance, and adherence to lab safety procedures.


  • B.S./M.S. in Biochemistry, Chemistry, Biology, Bioengineering, Chemical Engineering, Pharmaceutical Sciences, or a related discipline 0-2 years of academic or biotech/pharma laboratory experience.
  • Experience with mammalian and/or bacterial protein expression; experience in aseptic technique.
  • Familiarity with basic molecular biology techniques and plasmid preparation protocols.
  • Familiarity with protein purification strategies, and biochemical and analytical methods.
  • Experience with automated protein purification instrumentation is preferred.
  • Experience with standard biochemical analysis methods, including SDS-PAGE, ELISA and western blotting.
  • Strong interpersonal and presentation skills.
  • Desire to work in an accelerated environment, experimental rigor, strong organizational skills, and excellent attention to detail.
  • Able to work well independently and as an integral part of a team.

Repertoire is committed towards social responsibility and developing an inclusive culture. Much as the power of the immune system lies in the diversity of T and B cells, our work requires the creativity and ingenuity of a diverse workforce.  We believe in actively pursuing equity in all facets of the work experience at Repertoire.  We will continue to educate ourselves about the inequities and barriers present in our society and take action as a company where we can make a difference.

Repertoire is proud to be an Equal Opportunity Employer.