Scientist II/Senior Scientist, Cellular Immunology

Research & Development Cambridge, Massachusetts


Description

Company Overview

Repertoire Immune Medicines is a clinical-stage biotechnology company working to unlock and direct the remarkable power of the human immune system to prevent or treat cancer, as well as autoimmune and infectious disease.  The company is founded on the belief that the repertoire of T cell receptor (TCR)-antigen codes that drive health and disease represents one of the greatest opportunities for innovation in medical science.  Repertoire scientists created and developed the DECODE and DEPLOY suite of technologies, which allow in-depth characterization of TCR-antigen pairs, and the ability to rationally design and develop novel targeted immune medicines.

From its sites in Cambridge, Mass., and Zurich, Switzerland, Repertoire’s team of more than 120 is creating a new category of immune medicines enabled from its DECODE discovery platform.  The company’s first product in the clinic is a novel multiclonal T cell therapy consisting of T cells derived from the peripheral blood, activated against a curated set of antigens and armed with a proprietary cytokine.  Repertoire is in clinical-stage immuno-oncology development today, targeting metastatic solid tumors.  In the field of autoimmunity, Repertoire is currently using its proprietary platform to discover novel epitopes and TCRs from patients with type 1 diabetes.  However, we are currently expanding our remit into other autoimmune disorders.  The company is also using its DECODE platform to discover infectious disease antigens and responding T cell clones.

Role Overview

Repertoire is looking for an experienced and independent Scientist/Scientist II, Cellular Immunology to join the Preclinical Biology team within the Tolerance Platform group, which is responsible for developing antigen specific therapies for the treatment of autoimmune disease.  The successful candidate will take on a key role in the development of in vitro methodologies to evaluate the effects of drug candidates on primary human T cells and dendritic cells, to determine their mechanism of action, and ultimately to assist in bridging mouse pharmacology models to human biology.

Reporting to the Preclinical Biology team lead, the candidate will work closely with in vivo immunologists and collaborate with the Nanotech Product Engineering team.  Strong interpersonal, verbal and written communication skills, along with experimental rigor and scientific curiosity are key!  The ability to work in a fast-paced, matrixed team environment, and adaptability to evolving priorities are a must.

Key Responsibilities

  • Work as a key member of a tightly integrated cross-functional Platform Discovery team.
  • Read the literature, design experiments, lead execution, analyze data, and communicate results to the team.
  • Develop and validate antigen-agnostic and antigen-specific human and mouse in vitro T cell and DC systems to characterize therapeutic drug candidates and validate antigen targets. 
  • Contribute to the optimization of mRNA payload designs for tolerance induction.
  • Contribute to ex vivo immune cell assays including co-culture and others.
  • Contribute to the development of novel in vivo models to extend the scope of pre-clinical investigations in autoimmune therapy.
  • Maintain and implement good laboratory practices including accurate laboratory notebook systems, instrument and reagent validation, and personnel safety precautions.

Qualifications/Experience

  • Ph.D. in Immunology, Autoimmune Biology, or adjacent disciplines.
  • 2-6 years postgraduate experience in biotech/pharma industry or academia (experience in industry is preferred).
  • Impeccable sterile technique and tissue culture skills.
  • Extensive experience with isolation and enrichment of various immune cell subsets from human and mouse samples including peripheral blood, organs, and tissue biopsies.
  • Extensive experience with culturing human and mouse T cells, dendritic cells, and other immune cell subsets including various transfection, activation, and differentiation protocols.
  • Extensive experience with human and mouse DC T cell co-culture approaches including antigen processing and presentation, MLR, killing, suppression, and other protocols.
  • Proficiency with multi-color flow cytometry (ICS, proliferation assays, tetramer staining, viability) and cytokine assays (ELISA, Luminex, ELISPOT).
  • Proficiency with protein analysis by (co-)immunoprecipitation, gel electrophoresis, and western blotting.
  • Molecular biology experience (molecular cloning, codon optimization) is preferred.
  • Strong leadership, personal organizational, multitasking, and communication skills.
  • Team player with the ability to work in a matrixed, fast-paced, and quickly changing environment.
  • Balances creativity and science with delivery on key milestones within defined timelines.

 

Repertoire is committed towards social responsibility and developing an inclusive culture.  Much as the power of the immune system lies in the diversity of T and B cells, our work requires the creativity and ingenuity of a diverse workforce.  We believe in actively pursuing equity in all facets of the work experience at Repertoire.  We will continue to educate ourselves about the inequities and barriers present in our society and take action as a company where we can make a difference.

Repertoire is proud to be an Equal Opportunity Employer.