Scientist/Sr. Scientist Protein Engineering #149

Research & Development Cambridge, Massachusetts


Repertoire is seeking a highly motivated and innovative scientist to join our immunotherapy discovery team. Primary responsibility will be the engineering of next-generation biologics and optimization of antibodies and cytokines. This is a unique opportunity to collaborate with colleagues in Discovery and Early Development teams to directly contribute to biotherapeutic discovery campaigns. In-depth understanding of antibody and protein structure-function relationships and in vitro display technologies (e.g. yeast display) will be key to this position. The ideal candidate will be highly proficient in the design of protein/antibody constructs, advanced molecular biology techniques, protein biochemical characterization, and cell biology analyses.

Key Responsibilities Include:

  • Integral member of highly collaborative team utilizing yeast display and rational protein engineering to develop novel biotherapeutic modalities for immune therapy
  • Apply expertise in protein and antibody structure/function, molecular biology, alternative formats (i.e. conjugates, bispecifics, etc.) ¸ and display technologies (e.g. yeast display) to biotherapeutic discovery and optimization across multiple projects
  • Lead execution of protein engineering and antibody discovery/optimization efforts such as humanization, affinity maturation, specificity modulation, and platform improvements
  • Perform biophysical characterization such as binding kinetics, protein stability, and specificity; cell-based binding and functional analyses using flow cytometry
  • Maintain a timely and accurate laboratory record of all activities
  • Present results regularly at team meetings and drive data-driven decisions

Minimum Qualifications:

  • PhD in molecular bioengineering, chemical engineering, biochemistry, structural biology, or a related discipline is required with at least 4+ years of post-doctoral training, at least 8 years’ experience (including 4+ years industry experience) required for senior scientist
  • Experience with antibody and biotherapeutic discovery and a strong working knowledge of protein and antibody structure/function
  • Strong expertise in protein engineering display technologies, preferably yeast surface display, are required
  • Must have strong expertise in wide range of molecular biology techniques including molecular cloning, construct design, Gibson assembly and sequence analysis
  • High proficiency with protein engineering methodologies, including mutagenesis, library design, and protein display technology (ideally yeast display)
  • Protein analytic capabilities, including protein-protein interactions (cellular binding, SPR, Octet), biochemical analyses (affinity chromatography, SEC, IEX, DLS, thermal stability)
  • Familiarity with protein modeling tools such as Coot and Rosetta and standard bioinformatic tools (IMGT, Expasy, BLAST)
  • Excellent written and oral communication skills
  • Strong track record of scientific accomplishments as demonstrated through publications in highly regarded journals and/or intellectual property generation

Preferred Qualifications:

  • Antibody structure-function and humanization experience highly desired
  • Experience with multicolor flow cytometry is preferred
  • Knowledge of immune oncology space is desirable
  • Understanding of cytokine/receptor biology and advanced cytokine engineering strategies
  • R&D experience in an industry setting with demonstrated successes in biologics drug discovery