Senior Scientist, T Cell Characterization

Research & Development Cambridge, Massachusetts


Description

Position overview:

We are seeking a highly motivated Scientist / Senior Scientist to work in the Cell Therapy Analytical Research team, which is responsible for developing and deploying best-in-class methods to characterize antigen-specific T cells.  This is an opportunity to contribute to the characterization and influence the development of our PRIME T cell therapy platform, a novel class of adoptive T cell immunotherapies that combine endogenous T cells and engineered immunomodulators.  Working with a dedicated team of scientists and research associates, the successful candidate will contribute to cross-functional efforts to characterize antigen-specificity and function of T cells in phenotypically heterogeneous, multi-clonal T cell populations derived from clinical samples.  The candidate will be responsible for development of novel analytical methodologies using Repertoire’s state of the art single-cell sequencing and tetramer library tools, as well as the refinement and deployment of classical immunological assays such as flow cytometry, ELISpot, and cytotoxicity to further process and product development goals.  As part of a multi-disciplinary team, the candidate will work closely with the departments of Cell Therapy Discovery, Translational Research, Discovery Immunology, Antigen and Product Discovery, CMC Analytical Development and Computational Sciences to advance the PRIME platform through clinical development.

The ideal candidate will have relevant experience related to in vitro characterization of human T cells, including expertise in complex immunological methodologies such as immunophenotyping via flow cytometry, FACS, Luminex, RNA sequencing, TCR receptor repertoire analysis, as well as functional assays such as cytokine secretion and cytotoxicity, and immune monitoring methods such as ELISpot.  The successful candidate will be motivated by defining and answering complex biological questions, as well as by working with the team to drive projects and programs forward.  Strong interpersonal, verbal and written communication skills along with experimental rigor and scientific curiosity are key!  The ability to work in a fast-paced, matrixed team environment, and adaptability to evolving priorities are a must.

Key Responsibilities:

  • Design and lead the execution of complex, experimentally driven projects and initiatives related to characterization of antigen specific T cell products. Contribute to development of regulatory strategy as relates to extended product characterization.
  • Develop and deploy novel and classical T cell immunoassays, including antigen-specific T cell clonotype and functional analysis of CD8 and CD4 T cells.
  • Collaborate with molecular biology teams on applications development of Repertoire’s single cell platform and tetramer library tools for antigen specific clone identification.
  • Collaborate with Discovery, CMC and Translational Research teams on deployment of antigen specific T cell characterization assays to characterize the PRIME manufacturing process, GMP cell products, and clinical patient samples.
  • Represent the T cell characterization team on cross-functional project and program teams.
  • Analyze, summarize, and communicate team leadership and project teams. Document work in ELN, and author project summaries as needed.
  • Provide scientific, project and laboratory leadership to junior scientific staff. Lead by example with lab safety.

 

 

Minimum Qualifications:

  • MS or PhD in Immunology, Cell Biology, Molecular Biology, Bioengineering, or a related field.
  • Minimum of 3 years of biotech/pharma experience post PhD, or MS plus equivalent experience.
  • Subject matter expertise in general in vitro cell culture / immunology techniques and complex immunological methodologies such as cytometry and immunoassay methodologies such as flow/FACS, ICS, ELISpot, ELISA, Luminex, cytotoxicity.
  • Knowledge of antigen presenting cell biology, antigen presenting cell / T cell interactions, and peptide/MHC/TCR interactions is desired.
  • Experience with sequencing approaches to T cell characterization, including RNAseq and TCR clonotype analysis (bulk or single cell) is a plus.
  • Ability to identify, troubleshoot and solve issues associated with complex immunological methods.
  • Strong organizational skills, excellent attention to detail.
  • Proven track record of being personable, energetic, accountable, and an effective communicator
  • Must work well independently as well as an integral part of the team
  • Ability to work in a fast-paced environment.